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ThinkStock/Erin McCarthy
ThinkStock/Erin McCarthy

Can We Determine the Sex of a Dinosaur?

ThinkStock/Erin McCarthy
ThinkStock/Erin McCarthy

On August 12, 1990, the most complete Tyrannosaurus rex skeleton ever discovered was unearthed in the badlands of South Dakota by paleontologists Peter Larson and Susan Hendrickson. The animal was nicknamed “Sue” in honor of the latter scientist and, after a lengthy legal battle, her remains were purchased for the hefty sum of $8.4 million by the Chicago Field Museum. Today, she's easily become the world's most famous dinosaur. But what if “Sue” wasn't actually a female?

“We'd given the T. rex that name and always referred to it as 'she' or 'her',” said Larson, “But even at the site, people were asking me 'What if she's a male?' I'd say that we could always point to that Johnny Cash song 'A Boy Named Sue.'”

Country classics notwithstanding, can paleontologists accurately determine the sex of a long-dead dinosaur?

The short answer is yes ... and no.

While Sue's gender remains very much in question, comparatively recent research has shown that at least some dinosaur specimens may be sexed with reasonable certainty by modern scientists. The riddle's solution may very well lie in the bones of present-day dinos—also known as “birds."

Producing eggs is a very taxing process for expectant avian mothers, as the calcium in their shells is biologically expensive to build. When eggs start forming in the womb of a gravid bird, her body releases a heightened level of estrogen. This triggers the growth of what's known as “medullary bone” inside of her hollow leg and arm bones. The specialized medullary bone tissue provides much-needed calcium for the growing eggshells and lingers until their completion, at which point it disappears.

In 2005, paleontologists at North Carolina State University and Montana State University announced that they'd discovered medullary tissue within the limb bones of a T. rex specimen, suggesting that the animal was A) female and B) gravid at the time of its death.

The approach is hardly fool-proof: Only the bones of pregnant female dinosaurs would show any sign of medullary tissue, rendering the technique useless for deducing the sex of the vast majority of dinosaur skeletons. Nevertheless, the findings have excited a number of paleontologists, including one international team which sought to deduce the link between gender and this tell-tale tissue in a group of well-preserved fossilized birds this past February.

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Big Questions
How Are Speed Limits Set?
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When driving down a road where speed limits are oppressively low, or high enough to let drivers get away with reckless behavior, it's easy to blame the government for getting it wrong. But you and your fellow drivers play a bigger a role in determining speed limits than you might think.

Before cities can come up with speed limit figures, they first need to look at how fast motorists drive down certain roads when there are no limitations. According to The Sacramento Bee, officials conduct speed surveys on two types of roads: arterial roads (typically four-lane highways) and collector streets (two-lane roads connecting residential areas to arterials). Once the data has been collected, they toss out the fastest 15 percent of drivers. The thinking is that this group is probably going faster than what's safe and isn't representative of the average driver. The sweet spot, according to the state, is the 85th percentile: Drivers in this group are thought to occupy the Goldilocks zone of safety and efficiency.

Officials use whatever speed falls in the 85th percentile to set limits for that street, but they do have some wiggle room. If the average speed is 33 mph, for example, they’d normally round up to 35 or down to 30 to reach the nearest 5-mph increment. Whether they decide to make the number higher or lower depends on other information they know about that area. If there’s a risky turn, they might decide to round down and keep drivers on the slow side.

A road’s crash rate also comes into play: If the number of collisions per million miles traveled for that stretch of road is higher than average, officials might lower the speed limit regardless of the 85th percentile rule. Roads that have a history of accidents might also warrant a special signal or sign to reinforce the new speed limit.

For other types of roads, setting speed limits is more of a cut-and-dry process. Streets that run through school zones, business districts, and residential areas are all assigned standard speed limits that are much lower than what drivers might hit if given free rein.

Have you got a Big Question you'd like us to answer? If so, let us know by emailing us at bigquestions@mentalfloss.com.

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Big Questions
Do Bacteria Have Bacteria?
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Drew Smith:

Do bacteria have bacteria? Yes.

We know that bacteria range in size from 0.2 micrometers to nearly one millimeter. That’s more than a thousand-fold difference, easily enough to accommodate a small bacterium inside a larger one.

Nothing forbids bacteria from invading other bacteria, and in biology, that which is not forbidden is inevitable.

We have at least one example: Like many mealybugs, Planococcus citri has a bacterial endosymbiont, in this case the β-proteobacterium Tremblaya princeps. And this endosymbiont in turn has the γ-proteobacterium Moranella endobia living inside it. See for yourself:

Fluorescent In-Situ Hybridization confirming that intrabacterial symbionts reside inside Tremblaya cells in (A) M. hirsutus and (B) P. marginatus mealybugs. Tremblaya cells are in green, and γ-proteobacterial symbionts are in red. (Scale bar: 10 μm.)
Fluorescent In-Situ Hybridization confirming that intrabacterial symbionts reside inside Tremblaya cells in (A) M. hirsutus and (B) P. marginatus mealybugs. Tremblaya cells are in green, and γ-proteobacterial symbionts are in red. (Scale bar: 10 μm.)

I don’t know of examples of free-living bacteria hosting other bacteria within them, but that reflects either my ignorance or the likelihood that we haven’t looked hard enough for them. I’m sure they are out there.

Most (not all) scientists studying the origin of eukaryotic cells believe that they are descended from Archaea.

All scientists accept that the mitochondria which live inside eukaryotic cells are descendants of invasive alpha-proteobacteria. What’s not clear is whether archeal cells became eukaryotic in nature—that is, acquired internal membranes and transport systems—before or after acquiring mitochondria. The two scenarios can be sketched out like this:


The two hypotheses on the origin of eukaryotes:

(A) Archaezoan hypothesis.

(B) Symbiotic hypothesis.

The shapes within the eukaryotic cell denote the nucleus, the endomembrane system, and the cytoskeleton. The irregular gray shape denotes a putative wall-less archaeon that could have been the host of the alpha-proteobacterial endosymbiont, whereas the oblong red shape denotes a typical archaeon with a cell wall. A: archaea; B: bacteria; E: eukaryote; LUCA: last universal common ancestor of cellular life forms; LECA: last eukaryotic common ancestor; E-arch: putative archaezoan (primitive amitochondrial eukaryote); E-mit: primitive mitochondrial eukaryote; alpha:alpha-proteobacterium, ancestor of the mitochondrion.

The Archaezoan hypothesis has been given a bit of a boost by the discovery of Lokiarcheota. This complex Archaean has genes for phagocytosis, intracellular membrane formation and intracellular transport and signaling—hallmark activities of eukaryotic cells. The Lokiarcheotan genes are clearly related to eukaryotic genes, indicating a common origin.

Bacteria-within-bacteria is not only not a crazy idea, it probably accounts for the origin of Eucarya, and thus our own species.

We don’t know how common this arrangement is—we mostly study bacteria these days by sequencing their DNA. This is great for detecting uncultivatable species (which are 99 percent of them), but doesn’t tell us whether they are free-living or are some kind of symbiont. For that, someone would have to spend a lot of time prepping environmental samples for close examination by microscopic methods, a tedious project indeed. But one well worth doing, as it may shed more light on the history of life—which is often a history of conflict turned to cooperation. That’s a story which never gets old or stale.

This post originally appeared on Quora. Click here to view.

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