Why Some Civil War Soldiers Glowed in the Dark

By the spring of 1862, a year into the American Civil War, Major General Ulysses S. Grant had pushed deep into Confederate territory along the Tennessee River. In early April, he was camped at Pittsburg Landing, near Shiloh, Tennessee, waiting for Maj. Gen. Don Carlos Buell’s army to meet up with him.

On the morning of April 6, Confederate troops based out of nearby Corinth, Mississippi, launched a surprise offensive against Grant’s troops, hoping to defeat them before the second army arrived. Grant’s men, augmented by the first arrivals from the Ohio, managed to hold some ground, though, and establish a battle line anchored with artillery. Fighting continued until after dark, and by the next morning, the full force of the Ohio had arrived and the Union outnumbered the Confederates by more than 10,000.

The Union troops began forcing the Confederates back, and while a counterattack stopped their advance it did not break their line. Eventually, the Southern commanders realized they could not win and fell back to Corinth until another offensive in August (for a more detailed explanation of the battle, see this animated history).

All told, the fighting at the Battle of Shiloh left more than 16,000 soldiers wounded and more 3,000 dead, and neither federal or Confederate medics were prepared for the carnage.

The bullet and bayonet wounds were bad enough on their own, but soldiers of the era were also prone to infections. Wounds contaminated by shrapnel or dirt became warm, moist refuges for bacteria, which could feast on a buffet of damaged tissue. After months marching and eating field rations on the battlefront, many soldiers’ immune systems were weakened and couldn’t fight off infection on their own. Even the army doctors couldn’t do much; microorganisms weren’t well understood and the germ theory of disease and antibiotics were still a few years away. Many soldiers died from infections that modern medicine would be able to nip in the bud.

A Bright Spot

Some of the Shiloh soldiers sat in the mud for two rainy days and nights waiting for the medics to get around to them. As dusk fell the first night, some of them noticed something very strange: their wounds were glowing, casting a faint light into the darkness of the battlefield. Even stranger, when the troops were eventually moved to field hospitals, those whose wounds glowed had a better survival rate and had their wounds heal more quickly and cleanly than their unilluminated brothers-in-arms. The seemingly protective effect of the mysterious light earned it the nickname “Angel’s Glow.”

In 2001, almost one hundred and forty years after the battle, seventeen-year-old Bill Martin was visiting the Shiloh battlefield with his family. When he heard about the glowing wounds, he asked his mom - a microbiologist at the USDA Agricultural Research Service who had studied luminescent bacteria that lived in soil - about it.

“So you know, he comes home and, 'Mom, you're working with a glowing bacteria. Could that have caused the glowing wounds?’” Martin told Science Netlinks. “And so, being a scientist, of course I said, ‘Well, you can do an experiment to find out.’”

And that’s just what Bill did.

He and his friend, Jon Curtis, did some research on both the bacteria and the conditions during the Battle of Shiloh. They learned that Photorhabdus luminescens, the bacteria that Bill’s mom studied and the one he thought might have something to do with the glowing wounds, live in the guts of parasitic worms called nematodes, and the two share a strange lifecycle. Nematodes hunt down insect larvae in the soil or on plant surfaces, burrow into their bodies, and take up residence in their blood vessels. There, they puke up the P. luminescens bacteria living inside them. Upon their release, the bacteria, which are bioluminescent and glow a soft blue, begin producing a number of chemicals that kill the insect host and suppress and kill all the other microorganisms already inside it. This leaves P. luminescens and their nematode partner to feed, grow and multiply without interruptions.

As the worms and the bacteria eat and eat and the insect corpse is more or less hollowed out, the nematode eats the bacteria. This isn’t a double cross, but part of the move to greener pastures. The bacteria re-colonize the nematode’s guts so they can hitch a ride as it bursts forth from the corpse in search of a new host.

The next meal shouldn’t be hard to find either, since P. luminescens already sent them an invitation to the party. Just before they got got back in their nematode taxi, P. luminescens were at critical mass in the insect corpse, and scientists think that that many glowing bacteria attract other insects to the body and make the nematode’s transition to a new host much easier.

A Good Light

Looking at historical records of the battle, Bill and Jon figured out that the weather and soil conditions were right for both P. luminescens and their nematode partners. Their lab experiments with the bacteria, however, showed that they couldn’t live at human body temperature, making the soldiers’ wounds an inhospitable environment. Then they realized what some country music fans already knew: Tennessee in the spring is green and cool. Nighttime temperatures in early April would have been low enough for the soldiers who were out there in the rain for two days to get hypothermia, lowering their body temperature and giving P. luminescens a good home.

Based on the evidence for P. luminescens’s presence at Shiloh and the reports of the strange glow, the boys concluded that the bacteria, along with the nematodes, got into the soldiers’ wounds from the soil. This not only turned their wounds into night lights, but may have saved their lives. The chemical cocktail that P. luminescens uses to clear out its competition probably helped kill off other pathogens that might have infected the soldiers’ wounds. Since neither P. luminescens nor its associated nematode species are very infectious to humans, they would have soon been cleaned out by the immune system themselves (which is not to say you should be self-medicating with bacteria; P. luminescens infections can occur, and can result in some nasty ulcers). The soldiers shouldn’t have been thanking the angels so much as the microorganisms.

As for Bill and Jon, their study earned them first place in team competition at the 2001 Intel International Science and Engineering Fair.

Where Exactly Is Anne Boleyn's Body?

Hulton Archive/Getty Images
Hulton Archive/Getty Images

Anne Boleyn had a pretty rough 1536. First, a pregnant Anne discovered her husband was having an affair with Jane Seymour, one of her ladies in waiting. Some believe the shock and betrayal caused Anne to suffer a miscarriage in early February—and at least one report says it was the boy Henry VIII so desperately wanted. The birth of a healthy baby boy probably would have saved Anne’s life, but since she was unable to produce a male heir to the throne, her husband decided to simply replace her. Anne found herself imprisoned in the Tower of London on May 2, accused of adultery, incest, and high treason. Her marriage was annulled on May 17, and she was relieved of her head on May 19.

To add insult to all of this injury, no one bothered to give Anne a proper burial. Though the execution itself was meticulously planned, it hadn't occurred to anyone that there was no coffin until after Anne’s head rolled. After rummaging around the grounds, someone eventually scrounged up an old arrow chest to cram the corpse into.

She and her brother were then buried in an unmarked grave in front of the altar at St. Peter’s ad Vincula, within the Tower of London, and then completely forgotten about for the next 300-plus years. It wasn’t until Tower repairs in 1876 that Anne resurfaced—maybe.

Bones were discovered under the altar during the renovations, and based on the circumstantial evidence of an arrow chest coffin, bones belonging to a slender woman between the ages of 25 and 35, and a decapitated head, it was assumed that the remains belonged to Anne. However, Henry VIII disposed of his fifth wife Katherine Howard in the exact same manner, and had her corpse thrown in with the pile of bodies accumulating under the altar. Still other women were decapitated and buried in the same place, including Margaret Pole, Countess of Salisbury; Lady Jane Grey; and Lady Rochford.

Despite the fact that five headless women were buried there at one point, only four bodies were uncovered. The remains of Katherine Howard had seemingly disappeared, perhaps due to the quicklime found in the graves. Regardless of the uncertainty, Queen Victoria had the bodies exhumed and placed in individual coffins. A plaque with the name of the person thought to be inside was affixed to each coffin, and each one was given a proper reburial underneath the altar.

Is it really Anne Boleyn who lies beneath, or did workers really find someone else, giving credence to the theory that Anne Boleyn’s relatives had her body secretly reburied elsewhere? Unless DNA testing is performed on the remains, we’ll probably never know.

Updated for 2019.

The Very Real Events That Inspired Game of Thrones's Red Wedding

Peter Graham's After the Massacre of Glencoe
Peter Graham's After the Massacre of Glencoe
Peter Graham, Google Cultural Institute, Public Domain, Wikimedia Commons

Ask any Game of Thrones fan to cite a few of the show's most shocking moments, and the so-called "Red Wedding" from season 3's "The Rains of Castamere" episode will likely be at the top of their list. The events that unfolded during the episode shocked fans because of their brutality, but what might be even more surprising to know is that the episode was based on very real events.

Author George R.R. Martin has said that the inspiration for the matrimonial bloodbath is based on two dark events in Scottish history: the Black Dinner of 1440 and 1692's Massacre of Glencoe. “No matter how much I make up, there’s stuff in history that’s just as bad, or worse,” Martin told Entertainment Weekly in 2013. And he’s absolutely right. See for yourself.

The Massacre of Glencoe

The West Highland Way in 2005, view from the summit of the Devil's Staircase looking south over the east end of Glen Coe, towards Buachaille Etive Mòr with Creise and Meall a' Bhuiridh beyond
Colin Souza, Edited by Dave Souza, CC BY-SA 2.5, Wikimedia Commons

In 1691, all Scottish clans were called upon to renounce the deposed King of Scotland, James VII, and swear allegiance to King William of Orange (of William and Mary fame). The chief of each clan had until January 1, 1692, to provide a signed document swearing an oath to William. The Highland Clan MacDonald had two things working against them here. First of all, the Secretary of State, John Dalrymple, was a Lowlander who loathed Clan MacDonald. Secondly, Clan MacDonald had already sworn an oath to James VII and had to wait on him to send word that they were free to break that oath.

Unfortunately, it was December 28 before a messenger arrived with this all-important letter from the former king. That gave Maclain, the chief of the MacDonald clan, just three days to get the newly-signed oath to the Secretary of State.

Maclain was detained for days when he went through Inveraray, the town of the rival Clan Campbell, but still managed to deliver the oath, albeit several days late. The Secretary of State’s legal team wasn't interested in late documents. They rejected the MacDonalds's sworn allegiance to William, and set plans in place to cut the clan down, “root and branch.”

In late January or early February, 120 men under the command of Captain Robert Campbell arrived at the MacDonalds's in Glencoe, claiming to need shelter because a nearby fort was full. The MacDonalds offered their hospitality, as was custom, and the soldiers stayed there for nearly two weeks before Captain Drummond arrived with instructions to “put all to the sword under seventy.”

After playing cards with their victims and wishing them goodnight, the soldiers waited until the MacDonalds were asleep ... then murdered as many men as they could manage. In all, 38 people—some still in their beds—were killed. At least 40 women and children escaped, but fleeing into a blizzard blowing outside as their houses burned down meant that they all died of exposure.

The massacre was considered especially awful because it was “Slaughter Under Trust.” To this day, the door at Clachaig Inn in Glen Coe has a sign on the door that says "No hawkers or Campbells."

The Black Dinner

In November of 1440, the newly-appointed 6th Earl of Douglas, who was just 16, and his little brother David, were invited to join the 10-year-old King of Scotland, James II, for dinner at Edinburgh Castle. But it wasn’t the young King who had invited the Douglas brothers. The invitation had been issued by Sir William Crichton, Chancellor of Scotland, who feared that the Black Douglas (there was another clan called the Red Douglas) were growing too powerful.

As legend has it, the children were all getting along marvelously, enjoying food, entertainment and talking until the end of the dinner, when the head of a black bull was dropped on the table, symbolizing the death of the Black Douglas. The two young Douglases were dragged outside, given a mock trial, found guilty of high treason, and beheaded. It’s said that the Earl pleaded for his brother to be killed first so that the younger boy wouldn’t have to witness his older brother’s beheading.

Sir Walter Scott wrote this of the horrific event:

"Edinburgh Castle, toune and towre,
God grant thou sink for sin!
And that e'en for the black dinner
Earl Douglas gat therein."

This article has been updated for 2019.

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